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+1-888.733.6849
+1-617.299.7367 (Int’l)
Reactivity | Human Mouse Rat |
Tested applications | WB IHC |
Recommended Dilution | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
Calculated MW | 76kDa |
Observed MW | Refer to Figures |
Immunogen | Recombinant protein of human NCL |
Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Synonym | C23; |
Western blot analysis of extract of various cells, using NCL antibody.
Immunohistochemistry of paraffin-embedded human gastric using NCL antibody at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using NCL antibody at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using NCL antibody at dilution of 1:200 (40x lens).
Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20.
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