|Reactivity||Human Mouse Rat|
|Tested applications||WB IHC IF|
|Recommended Dilution||WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200|
|Observed MW||Refer to figures|
|Immunogen||Recombinant protein of human NME2|
|Storage Buffer||Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.|
|Synonym||PUF; NDKB; NDPKB; NM23B; NDPK-B; NM23-H2;|
Western blot analysis of extracts of various cell lines, using NME2 antibody.
Immunohistochemistry of paraffin-embedded human kidney using NME2 antibody at dilution of 1:100 (x400 lens).
Immunohistochemistry of paraffin-embedded mouse kidney using NME2 antibody at dilution of 1:100 (x400 lens).
Immunofluorescence analysis of A549 cell using NME2 antibody. Blue: DAPI for nuclear staining.
Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by NME1) and 'B' (encoded by this gene) isoforms. Multiple alternatively spliced transcript variants have been found for this gene. Read-through transcription from the neighboring upstream gene (NME1) generates naturally-occurring transcripts (NME1-NME2) that encode a fusion protein comprised of sequence sharing identity with each individual gene product.
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