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Reactivity | Human Mouse |
Tested applications | WB IHC ICC IF IP FC |
Recommended Dilution | WB 1:500 - 1:2000 IHC 1:50 - 1:200 ICC 1:50 - 1:200 IF 1:50 - 1:100 IP 1:20 - 1:50 FC 1:20 - 1:50 |
Calculated MW | 125 kDa |
Observed MW | Refer to figures |
Immunogen | Recombinant protein of human NOTCH1 |
Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Synonym | hN1; AOS5; TAN1; AOVD1; |
Western blot analysis of extracts of HepG2 cells, using NOTCH1 antibody.
Immunohistochemistry of paraffin-embedded mouse lung using NOTCH1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung using NOTCH1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human spleen using NOTCH1 antibody at dilution of 1:100 (40x lens).
Immunofluorescence
This gene encodes a member of the NOTCH family of proteins. Members of this Type I transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple different domain types. Notch signaling is an evolutionarily conserved intercellular signaling pathway that regulates interactions between physically adjacent cells through binding of Notch family receptors to their cognate ligands. The encoded preproprotein is proteolytically processed in the trans-Golgi network to generate two polypeptide chains that heterodimerize to form the mature cell-surface receptor. This receptor plays a role in the development of numerous cell and tissue types. Mutations in this gene are associated with aortic valve disease, Adams-Oliver syndrome, T-cell acute lymphoblastic leukemia, chronic lymphocytic leukemia, and head and neck squamous cell carcinoma.
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