|Reactivity||Human Mouse Rat|
|Tested applications||WB IHC ICC IF IP|
|Recommended Dilution||WB 1:500 - 1:2000
IHC 1:50 - 1:200
ICC 1:20 - 1:100
IF 1:50 - 1:200
IP 1:20 - 1:100|
|Observed MW||Refer to Figures|
|Immunogen||Recombinant protein of human PARK7|
|Storage Buffer||Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.|
Western blot analysis of extracts of various cell lines, using PARK7 antibody.
Immunofluorescence analysis of HeLa cell using PARK7 antibody. Blue: DAPI for nuclear staining.
Parkinson's disease (PD) is characterized by the presence of Lewy bodies (intracellular inclusions) and by the loss of dopaminergic neurons. Research studies have shown that mutations in α-synuclein, Parkin, and DJ-1 are linked to PD (1). α-synuclein is a major component of the aggregates found in Lewy bodies. Parkin is involved in protein degradation through the ubiquitin-proteasome pathway, and investigators have shown that mutations in Parkin cause early onset of PD (1). Loss-of-function mutations in DJ-1 cause early onset of PD, but DJ-1 is associated with multiple functions: it cooperates with Ras to increase cell transformation, it positively regulates transcription of the androgen receptor, and it may function as an indicator of oxidative stress (2-5). Dopamine D2 receptor-mediated functions are greatly impaired in DJ-1 (-/-) mice, resulting in reduced long-term depression (6).
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