|Tested applications||WB IHC|
|Recommended Dilution||WB 1:1000 - 1:3000
IHC 1:50 - 1:100|
|Observed MW||Refer to Figures|
|Immunogen||Recombinant protein of human PARP1|
|Storage Buffer||Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.|
|Synonym||PARP; PARP1; PPOL; ADPRT; ADPRT1; PARP1-1; ADPRT 1; pADPRT-1;|
Immunohistochemistry of paraffin-embedded mouse stomach using PARP1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophageal cancer using PARP1 antibody at dilution of 1:100 (40x lens).
PARP1, a 116 kDa nuclear poly (ADP-ribose) polymerase, appears to be involved in DNA repair in response to environmental stress (1). This protein can be cleaved by many ICE-like caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP1, the cleavage occurs between Asp214 and Gly215, which separates the PARP1 amino-terminal DNA binding domain (24 kDa) from the carboxy-terminal catalytic domain (89 kDa) (2,4). PARP1 helps cells to maintain their viability; cleavage of PARP1 facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6).
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