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+1-617.299.7367 (Int’l)
Reactivity | Human Mouse Rat |
Tested applications | WB IHC |
Recommended Dilution | WB 1:500 - 1:2000 IHC 1:50 - 1:100 |
Calculated MW | 47kDa |
Observed MW | Refer to figures |
Immunogen | Recombinant protein of human PDK3 |
Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Synonym | CMTX6; GS1-358P8.4; |
Western blot analysis of extracts of various cells, using PDK3 antibody.
Immunohistochemistry of paraffin-embedded mouse lung using PDK3 antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry of paraffin-embedded rat brain using PDK3 antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry of paraffin-embedded rat kidney using PDK3 antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry of paraffin-embedded human liver injury using PDK3 antibody at dilution of 1:100 (x40 lens).
The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle, and thus is one of the major enzymes responsible for the regulation of glucose metabolism. The enzymatic activity of PDH is regulated by a phosphorylation/dephosphorylation cycle, and phosphorylation results in inactivation of PDH. The protein encoded by this gene is one of the three pyruvate dehydrogenase kinases that inhibits the PDH complex by phosphorylation of the E1 alpha subunit. This gene is predominantly expressed in the heart and skeletal muscles. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
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