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Reactivity | Human Mouse Rat |
Tested applications | WB IHC IF |
Recommended Dilution | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
Calculated MW | 63kDa |
Observed MW | Refer to Figures |
Immunogen | Recombinant protein of human PDLIM5 |
Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Synonym | L9; ENH; LIM; ENH1; |
Western blot analysis of extracts of various cell lines, using PDLIM5 antibody.
Immunohistochemistry of paraffin-embedded Mouse heart using PDLIM5 antibody at dilution of 1:100 (x400 lens).
Immunofluorescence analysis of U20S cell using PDLIM5 antibody. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cell using PDLIM5 antibody. Blue: DAPI for nuclear staining.
This gene encodes a member of a family of proteins that possess a 100-amino acid PDZ domain at the N terminus and one to three LIM domains at the C-terminus. This family member functions as a scaffold protein that tethers protein kinases to the Z-disk in striated muscles. It is thought to function in cardiomyocyte expansion and in restraining postsynaptic growth of excitatory synapses. Alternative splicing of this gene results in multiple transcript variants.
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