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Reactivity | Human Mouse Rat |
Tested applications | WB IHC IF |
Recommended Dilution | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
Calculated MW | 71kDa |
Observed MW | Refer to figures |
Immunogen | Recombinant protein of human PIAS1 |
Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Synonym | GBP; ZMIZ3; DDXBP1; GU/RH-II; |
Western blot analysis of extract of various cells, using PIAS1 antibody.
Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cell using PIAS1 antibody. Green: GFP-RNF168 fusion protein expression for DNA damage marker. Blue: DAPI for nuclear staining. RNF168 (GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.
Immunofluorescence analysis of U2OS cell using PIAS1 antibody. Blue: DAPI for nuclear staining.
This gene encodes a member of the mammalian PIAS
N/A