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Reactivity | Human Mouse Rat |
Tested applications | WB IHC IF |
Recommended Dilution | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
Calculated MW | 123kDa |
Observed MW | Refer to Figures |
Immunogen | Recombinant protein of human POLD1 |
Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Concentration | e |
Synonym | CDC2; MDPL; POLD; CRCS10; |
Western blot analysis of extracts of various cell lines, using POLD1 antibody.
Immunofluorescence analysis of U2OS cell using POLD1 antibody. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of A549 cell using POLD1 antibody.
Immunofluorescence analysis of U2OS cell using POLD1 antibody. Green:gamma H2AX (phospho S139) used for DNA damage marker. Blue: DAPI for nuclear staining. Cells were treated by UV-A laser.
This gene encodes the 125-kDa catalytic subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 6.
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