POLE3-Polyclonal Antibodies

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POLE3

Qty


Total
$220
Catalog #
A6469
Antibody Type
Polyclonal Antibody
Gene ID
54107
Swiss Prot
Q9NRF9
Size
Species
Rabbit
Isotype
IgG
Purity
Affinity purification
Additional Information
ReactivityHuman Mouse Rat
Tested applicationsWB IHC IF
Recommended DilutionWB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Calculated MW16kDa
Observed MWRefer to Figures
ImmunogenRecombinant protein of human POLE3
Storage BufferStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Synonymp17; YBL1; CHRAC17; CHARAC17;
Images
  • A6469: image 1

    Western blot analysis of extracts of MCF-7 cells, using POLE3 antibody.

  • A6469: image 2

    Immunofluorescence analysis of U2OS cell using POLE3 antibody.

  • A6469: image 3

    Immunofluorescence analysis of U2OS cell using POLE3 antibody. Green:gamma H2AX (phospho S139) used for DNA damage marker. Blue: DAPI for nuclear staining. Cells were treated by UV-A laser.

  • A6469: image 4

    Immunofluorescence analysis of U2OS cell using POLE3 antibody. Green:gamma H2AX (phospho S139) used for DNA damage marker. Blue: DAPI for nuclear staining. Cells were treated by UV-A laser.

Background

POLE3 is a histone-fold protein that interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. These histone-fold protein dimers combine within larger enzymatic complexes for DNA transcription, replication, and packaging.

Protocol

N/A

MSDS
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