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Reactivity | Human Mouse Rat |
Tested applications | WB IHC IF |
Recommended Dilution | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 |
Calculated MW | 16kDa |
Observed MW | Refer to Figures |
Immunogen | Recombinant protein of human POLE3 |
Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Synonym | p17; YBL1; CHRAC17; CHARAC17; |
Western blot analysis of extracts of MCF-7 cells, using POLE3 antibody.
Immunofluorescence analysis of U2OS cell using POLE3 antibody.
Immunofluorescence analysis of U2OS cell using POLE3 antibody. Green:gamma H2AX (phospho S139) used for DNA damage marker. Blue: DAPI for nuclear staining. Cells were treated by UV-A laser.
Immunofluorescence analysis of U2OS cell using POLE3 antibody. Green:gamma H2AX (phospho S139) used for DNA damage marker. Blue: DAPI for nuclear staining. Cells were treated by UV-A laser.
POLE3 is a histone-fold protein that interacts with other histone-fold proteins to bind DNA in a sequence-independent manner. These histone-fold protein dimers combine within larger enzymatic complexes for DNA transcription, replication, and packaging.
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