|Reactivity||Human Mouse Rat|
|Tested applications||WB IHC IF|
|Recommended Dilution||WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200|
|Observed MW||Refer to Figures|
|Immunogen||Recombinant protein of human PPP2R4|
|Storage Buffer||Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.|
|Synonym||PP2A; PR53; PTPA;|
Western blot analysis of extracts of MCF7 cell line, using PPP2R4 antibody.
Immunohistochemistry of paraffin-embedded rat brain using PPP2R4 antibody at dilution of 1:200 (x400 lens)
Immunohistochemistry of paraffin-embedded human kidney using PPP2R4 antibody at dilution of 1:200 (x400 lens)
Immunofluorescence analysis of U2OS cell using PPP2R4 antibody. Blue: DAPI for nuclear staining.
Protein phosphatase 2A is one of the four major Ser/Thr phosphatases and is implicated in the negative control of cell growth and division. Protein phosphatase 2A holoenzymes are heterotrimeric proteins composed of a structural subunit A, a catalytic subunit C, and a regulatory subunit B. The regulatory subunit is encoded by a diverse set of genes that have been grouped into the B/PR55, B'/PR61, and B''/PR72 families. These different regulatory subunits confer distinct enzymatic specificities and intracellular localizations to the holozenzyme. The product of this gene belongs to the B' family. This gene encodes a specific phosphotyrosyl phosphatase activator of the dimeric form of protein phosphatase 2A. Alternative splicing results in multiple transcript variants encoding different isoforms.
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