PRF1-Polyclonal Antibodies

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PRF1

Qty


Total
$220
Catalog #
A0093
Antibody Type
Polyclonal Antibody
Gene ID
5551
Swiss Prot
P14222
Size
Species
Rabbit
Isotype
IgG
Purity
Affinity purification
Additional Information
ReactivityHuman Mouse
Tested applicationsWB IHC IF
Recommended DilutionWB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Calculated MW61kDa
Observed MWRefer to Figures
ImmunogenRecombinant protein of human PRF1
Storage BufferStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentrationb
SynonymPRF1;FLH2;HPLH2;MGC65093;P1;PFN1;PFP
Images
  • A0093: image 1

    Western blot analysis of extracts of various cell lines, using PRF1 antibody.

  • A0093: image 2

    Immunofluorescence analysis of HeLa cell using PRF1 antibody. Blue: DAPI for nuclear staining.

Background

Granzymes are a family of serine proteases expressed by cytotoxic T lymphocytes and natural killer (NK) cells and are key components of immune responses to pathogens and transformed cells (1). Granzymes are synthesized as zymogens and are processed into mature enzymes by cleavage of a leader sequence. They are released by exocytosis in lysosome-like granules containing perforin, a membrane pore-forming protein. Granzyme B has the strongest apoptotic activity of all the granzymes as a result of its caspase-like ability to cleave substrates at aspartic acid residues thereby activating procaspases directly and cleaving downstream caspase substrates (2,3). Perforin is a pore-forming protein that facilitates the entry of cytotoxic serine proteases, such as granzymes, into target cells (4). Perforin is primarily expressed in cytotoxic T lymphocytes and NK cells.

Protocol

N/A

MSDS
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