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Reactivity | Human Mouse Rat |
Tested applications | WB IHC IF |
Recommended Dilution | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 |
Calculated MW | 38kDa |
Observed MW | Refer to figures |
Immunogen | Recombinant protein of human PRKAG1 |
Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Synonym | AMPKG; |
Western blot analysis of extracts of various cell lines, using PRKAG1 antibody.
Immunohistochemistry of paraffin-embedded human gastric using PRKAG1 antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry of paraffin-embedded rat brain using PRKAG1 antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry of paraffin-embedded mouse kidney using PRKAG1 antibody at dilution of 1:100 (x40 lens).
Immunofluorescence analysis of MCF7 cell using PRKAG1 antibody. Blue: DAPI for nuclear staining.
The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit is one of the gamma regulatory subunits of AMPK. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
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