PRKD1-Polyclonal Antibodies

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PRKD1

Qty


Total
$380
Catalog #
A0101
Antibody Type
Polyclonal Antibody
Gene ID
5587
Swiss Prot
Q15139
Size
Species
Rabbit
Isotype
IgG
Purity
Affinity purification
Additional Information
ReactivityHuman Mouse Rat
Tested applicationsWB
Recommended DilutionWB 1:500 - 1:2000
Calculated MW105kDa
Observed MWRefer to Figures
ImmunogenA synthetic peptide of human PRKD1
Storage BufferStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentrationq
SynonymPKC-MU;PKCM;PKD;PRKCM;Protein kinase C mu type antibody;Protein kinase D antibody;nPKC-D1 antibody, nPKC-mu antibody;
Images
  • A0101: image 1

    Western blot analysis of extracts of various cell lines, using PRKD1 antibody.

Background

PKC mu is a novel member of the subgroup of atypical protein kinase Cs (PKC). Deduced protein sequence shows strong homology to conserved domains of members of the PKC subfamily. In vitro phorbol ester binding studies and kinase assays with lysates of cells overexpressing PKC mu showed phorbol ester-independent kinase activity, autophosphorylation, and, in normal rat kidney (NRK) cells, predominant phosphorylation of a 30-kDa protein at serine residues. Data suggest a role of PKC mu in signal transduction pathways related to growth control. PKC mu is a cytosolic protein, which upon binding to the trans-Golgi network (TGN) regulates the fission of transport carriers specifically destined to the cell surface. Mutation of serines 744/748 to alanines in the activation loop of intact PKD inhibits its localization to the TGN. Moreover, anti-phospho-PKD antibody, which recognizes only the activated form of PKD, recognizes the TGN-bound PKD. Thus, activation of intact PKD is important for binding to the TGN. Results demonstrate that betagamma subunits of the heterotrimeric G proteins directly activates PKD by interacting with its pleckstrin homology domain.

Protocol

N/A

MSDS
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