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Reactivity | Human Mouse Rat |
Tested applications | WB IHC IF |
Recommended Dilution | WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100 |
Calculated MW | 97kDa |
Observed MW | Refer to Figures |
Immunogen | A synthetic peptide of human PROM1 |
Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Synonym | CD133 ;PROM1;PROM1 1;MSTP061;MCDR2;PROM1 1;6;7 |
Western blot analysis of extracts of SW620 cells, using PROM1 antibody.
Immunohistochemistry of paraffin-embedded human prostate using PROM1 antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry of paraffin-embedded human lung using PROM1 antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry of paraffin-embedded mouse brain using PROM1 antibody.
Immunohistochemistry of paraffin-embedded rat brain using PROM1 antibody.
Immunofluorescence analysis of U2OS cell using PROM1 antibody. Blue: DAPI for nuclear staining.
This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. Mutations in this gene have been shown to result in retinitis pigmentosa and Stargardt disease. Expression of this gene is also associated with several types of cancer. This gene is expressed from at least five alternative promoters that are expressed in a tissue-dependent manner. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]
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