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Reactivity | Human Mouse Rat |
Tested applications | WB IHC IF |
Recommended Dilution | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
Calculated MW | 78kDa |
Observed MW | Refer to Figures |
Immunogen | Recombinant protein of human PRPF3 |
Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Synonym | PRP3; RP18; HPRP3; Prp3p; HPRP3P; SNRNP90; |
Western blot analysis of extracts of various cell lines, using PRPF3 antibody.
Immunohistochemistry of paraffin-embedded Human gastric using PRPF3 antibody at dilution of 1:100 (x400 lens).
Immunohistochemistry of paraffin-embedded Human gastric cancer using PRPF3 antibody at dilution of 1:100 (x400 lens).
Immunohistochemistry of paraffin-embedded Human mammary gland using PRPF3 antibody at dilution of 1:100 (x400 lens).
Immunohistochemistry of paraffin-embedded liver cancer using PRPF3 antibody at dilution of 1:100 (x400 lens).
Immunohistochemistry of paraffin-embedded Mouse brain using PRPF3 antibody at dilution of 1:100 (x400 lens).
Immunohistochemistry of paraffin-embedded Rat brain using PRPF3 antibody at dilution of 1:100 (x400 lens).
Immunofluorescence analysis of U20S cell using PRPF3 antibody. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cell using PRPF3 antibody.
The removal of introns from nuclear pre-mRNAs occurs on complexes called spliceosomes, which are made up of 4 small nuclear ribonucleoprotein (snRNP) particles and an undefined number of transiently associated splicing factors. This gene product is one of several proteins that associate with U4 and U6 snRNPs. Mutations in this gene are associated with retinitis pigmentosa-18.
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