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Reactivity | Human Mouse Rat |
Tested applications | WB IHC |
Recommended Dilution | WB 1:500 - 1:1000 IHC 1:50 - 1:100 |
Calculated MW | 273kDa |
Observed MW | Refer to Figures |
Immunogen | A synthetic peptide of human PRPF8 |
Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Concentration | j |
Synonym | PRP8; RP13; HPRP8; PRPC8; SNRNP220; |
Western blot analysis of extracts of various cell lines, using PRPF8 antibody.
Immunohistochemistry of paraffin-embedded human esophagus using PRPF8 antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry of paraffin-embedded rat brain using PRPF8 antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry of paraffin-embedded mouse stomach using PRPF8 antibody at dilution of 1:100 (x40 lens).
Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is a component of both U2- and U12-dependent spliceosomes, and found to be essential for the catalytic step II in pre-mRNA splicing process. It contains several WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp8 protein. This gene is a candidate gene for autosomal dominant retinitis pigmentosa.
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