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Reactivity | Human Mouse Rat |
Tested applications | WB IHC IF |
Recommended Dilution | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 |
Calculated MW | 29kDa |
Observed MW | Refer to figures |
Immunogen | Recombinant protein of human PSMB10 |
Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Synonym | LMP10; MECL1; beta2i; |
Western blot analysis of extracts of THP-1 cells, using PSMB10 antibody.
Immunohistochemistry of paraffin-embedded rat brain using PSMB10 antibody at dilution of 1:200 (400x lens).
Immunohistochemistry of paraffin-embedded mouse brain using PSMB10 antibody at dilution of 1:200 (400x lens).
Immunofluorescence analysis of HeLa cell using PSMB10 antibody. Blue: DAPI for nuclear staining.
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. Proteolytic processing is required to generate a mature subunit. Expression of this gene is induced by gamma interferon, and this gene product replaces catalytic subunit 2 (proteasome beta 7 subunit) in the immunoproteasome.
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