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Reactivity | Human Mouse Rat |
Tested applications | WB IHC IF |
Recommended Dilution | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 |
Calculated MW | 43kDa |
Observed MW | Refer to figures |
Immunogen | Recombinant protein of human PSMD13 |
Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Synonym | S11; Rpn9; p40.5; HSPC027; |
Western blot analysis of extracts of various cell lines, using PSMD13 antibody.
Immunohistochemistry of paraffin-embedded human liver injury using PSMD13 antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry of paraffin-embedded mouse lung using PSMD13 antibody at dilution of 1:100 (x40 lens).
Immunofluorescence analysis of U20S cell using PSMD13 antibody. Blue: DAPI for nuclear staining.
The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. Two transcripts encoding different isoforms have been described.
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