|Reactivity||Human Mouse Rat|
|Tested applications||WB IHC|
|Recommended Dilution||WB 1:500 - 1:2000
IHC 1:100 - 1:200|
|Observed MW||Refer to figures|
|Immunogen||Recombinant protein of human PTTG1|
|Storage Buffer||Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.|
|Synonym||EAP1; PTTG; HPTTG; TUTR1;|
Western blot analysis of extract of various cells, using PTTG1 antibody.
Immunohistochemistry of paraffin-embedded human lung cancer using PTTG1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver using PTTG1 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using PTTG1 antibody at dilution of 1:100 (40x lens).
The encoded protein is a homolog of yeast securin proteins, which prevent separins from promoting sister chromatid separation. It is an anaphase-promoting complex (APC) substrate that associates with a separin until activation of the APC. The gene product has transforming activity in vitro and tumorigenic activity in vivo, and the gene is highly expressed in various tumors. The gene product contains 2 PXXP motifs, which are required for its transforming and tumorigenic activities, as well as for its stimulation of basic fibroblast growth factor expression. It also contains a destruction box (D box) that is required for its degradation by the APC. The acidic C-terminal region of the encoded protein can act as a transactivation domain. The gene product is mainly a cytosolic protein, although it partially localizes in the nucleus. Three transcript variants encoding the same protein have been found for this gene.
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