Phospho-Bad-S155-Monoclonal Antibodies

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Phospho-Bad-S155

Qty


Total
$300
Catalog #
AP0009
Antibody Type
Polyclonal Antibody
Gene ID
572
Swiss Prot
Q92934
Size
Species
Rabbit
Isotype
IgG
Purity
Affinity purification
Additional Information
ReactivityHuman
Tested applicationsWB IHC
Recommended DilutionWB 1:500 - 1:2000 IHC 1:50 - 1:200
Calculated MW18kDa
Observed MWRefer to Figures
ImmunogenA phospho specific peptide corresponding to residues surrounding S155 of human Bad
Storage BufferStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentrationbiq
SynonymBBC2; BCL2L8;
Images
  • AP0009: image 1

    Western blot analysis of extracts of various cells, using Phospho-Bad-S155 antibody.

Background

Bad is a proapoptotic member of the Bcl-2 family that promotes cell death by displacing Bax from binding to Bcl-2 and Bcl-xL (1,2). Survival factors, such as IL-3, inhibit the apoptotic activity of Bad by activating intracellular signaling pathways that result in the phosphorylation of Bad at Ser112 and Ser136 (2). Phosphorylation at these sites promotes binding of Bad to 14-3-3 proteins to prevent an association between Bad with Bcl-2 and Bcl-xl (2). Akt phosphorylates Bad at Ser136 to promote cell survival (3,4). Bad is phosphorylated at Ser112 both in vivo and in vitro by p90RSK (5,6) and mitochondria-anchored PKA (7). Phosphorylation of Ser155 in the BH3 domain by PKA plays a critical role in blocking the dimerization of Bad and Bcl-xL (8-10).

Protocol

N/A

MSDS
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