|Reactivity||Human Mouse Rat|
|Tested applications||WB IHC IF|
|Recommended Dilution||WB 1:500 - 1:1000
IHC 1:50 - 1:100
IF 1:50 - 1:100|
|Observed MW||Refer to Figures|
|Immunogen||A phospho specific peptide corresponding to residues surrounding S383 of human ELK1|
|Storage Buffer||Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.|
|Synonym||ELK1; ETS-domain protein Elk-1;|
Western blot analysis of extracts from HT29 cells untreated or treated with UV, using Phospho-ELK1-S383 antibody.
Immunofluorescence analysis of MCF7 cell using Phospho-ELK1-S383 antibody. Blue: DAPI for nuclear staining.
Elk-1 is a member of the Ets family of transcription factors and of the ternary complex factor (TCF) subfamily. Proteins of the TCF subfamily form a ternary complex by binding to the the serum response factor and the serum reponse element in the promoter of the c-fos proto-oncogene. The protein encoded by this gene is a nuclear target for the ras-raf-MAPK signaling cascade. lternatively spliced transcript variants encoding the same protein have been found for this gene.
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