Phospho-FAS-Y291 -Monoclonal Antibodies

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Phospho-FAS-Y291

Qty


Total
$300
Catalog #
AP0007
Antibody Type
Polyclonal Antibody
Gene ID
355
Swiss Prot
P25445
Size
Species
Rabbit
Isotype
IgG
Purity
Affinity purification
Additional Information
ReactivityHuman
Tested applicationsWB
Recommended DilutionWB 1:500 - 1:2000
Observed MWRefer to Figures
ImmunogenA phospho specific peptide corresponding to residues surrounding Y291 of human FAS
Storage BufferStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentrationbi
SynonymAPT1; CD95; FAS1; APO-1; FASTM; ALPS1A; TNFRSF6;
Images
  • AP0007: image 1

    Western blot analysis of extracts of various cell lines, using Phospho-FAS-Y291 antibody.

Background

Association of the receptor Fas with its ligand FasL triggers an apoptotic pathway that plays an important role in immune regulation, development, and progression of cancers (1,2). Loss of function mutation in either Fas (lpr mice) or FasL (gld mice) leads to lymphadenopathy and splenomegaly as a result of decreased apoptosis in CD4-CD8- T lymphocytes (3,4). FasL (CD95L, Apo-1L) is a type II transmembrane protein of 280 amino acids (runs at approximately 40 kDa upon glycosylation) that belongs to the TNF family, which also includes TNF-α, TRAIL, and TWEAK. Binding of FasL to its receptor triggers the formation of a death-inducing signaling complex (DISC) involving the recruitment of the adaptor protein FADD and caspase-8 (5). Activation of caspase-8 from this complex initiates a caspase cascade resulting in the activation of caspase-3 and subsequent cleavage of proteins leading to apoptosis. Unlike Fas, which is constitutively expressed by various cell types, FasL is predominantly expressed on activated T lymphocytes, NK cells, and at immune privileged sites (6). FasL is also expressed in several tumor types as a mechanism to evade immune surveillance (7). Similar to other members of the TNF family, FasL can be cleaved by metalloproteinases producing a 26 kDa trimeric soluble form (8,9).

Protocol

N/A

MSDS
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