Phospho-NOS3-S1177-Tag and Loading control Antibodies

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Phospho-NOS3-S1177

Qty


Total
$300
Catalog #
AP0421
Antibody Type
Polyclonal Antibody
Gene ID
4846
Swiss Prot
P29474
Size
Species
Rabbit
Isotype
IgG
Purity
Affinity purification
Additional Information
ReactivityHuman Mouse Rat
Tested applicationsWB IHC IF
Recommended DilutionWB 1:500 - 1:1000 IHC 1:50 - 1:100 IF 1:100 - 1:200
Calculated MW140kDa
Observed MWRefer to Figures
ImmunogenA phospho specific peptide corresponding to residues surrounding S1177 of human NOS3
Storage BufferStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentrationa
SynonymeNOS; ECNOS;
Images
  • AP0421: image 1

    Western blot analysis of extracts of various cell lines, using Phospho-NOS3-S1177 antibody.

  • AP0421: image 2

    Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Phospho-NOS3-S1177 antibody or the same antibody preincubated with blocking peptide(right).

  • AP0421: image 3

    Immunofluorescence staining of methanol-fixed HeLa cells using Phospho-NOS3-S1177 Antibody.

Background

Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene.

Protocol

N/A

MSDS
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