|Reactivity||Human Mouse Rat|
|Tested applications||WB IHC IF|
|Recommended Dilution||WB 1:500 - 1:1000
IHC 1:50 - 1:100
IF 1:100 - 1:200|
|Observed MW||Refer to Figures|
|Immunogen||A phospho specific peptide corresponding to residues surrounding S1177 of human NOS3|
|Storage Buffer||Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.|
Western blot analysis of extracts of various cell lines, using Phospho-NOS3-S1177 antibody.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Phospho-NOS3-S1177 antibody or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed HeLa cells using Phospho-NOS3-S1177 Antibody.
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene.
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