|Reactivity||Human Mouse Rat|
|Tested applications||WB IHC IF|
|Recommended Dilution||WB 1:500 - 1:2000
IHC 1:50 - 1:100
IF 1:100 - 1:200|
|Observed MW||Refer to Figures|
|Immunogen||A phospho specific peptide corresponding to residues surrounding S727 of human STAT1|
|Storage Buffer||Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.|
|Synonym||CANDF7; ISGF-3; STAT91;|
Western blot analysis of extracts from Hela cells, using Phospho-STAT1-S727 antibody.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using Phospho-STAT1-S727 antibody.
Immunofluorescence staining of methanol-fixed Hela cells, using Phospho-STAT1-S727 antibody.
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. Two alternatively spliced transcript variants encoding distinct isoforms have been described.
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