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| Reactivity | Human Mouse Rat |
| Tested applications | WB IHC IF |
| Recommended Dilution | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Calculated MW | 56kDa |
| Observed MW | Refer to Figures |
| Immunogen | Recombinant protein of human RAD18 |
| Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Concentration | el |
| Synonym | RNF73; |
Western blot analysis of extracts of various cell lines, using RAD18 antibody.
Immunofluorescence analysis of U20S cell using RAD18 antibody. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cell using RAD18 antibody. Blue: DAPI for nuclear staining. DNA damage by a UV-A laser.
Immunofluorescence analysis of GFP-RNF168 trangenic U2OS cell using RAD18 antibody. Green：GFP-RNF168 fusion protein expression for DNA damage marker.Blue: DAPI for nuclear staining.RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.
The protein encoded by this gene is highly similar to S. cerevisiae DNA damage repair protein Rad18. Yeast Rad18 functions through its interaction with Rad6, which is an ubiquitin-conjugating enzyme required for post-replication repair of damaged DNA. Similar to its yeast counterpart, this protein is able to interact with the human homolog of yeast Rad6 protein through a conserved ring-finger motif. Mutation of this motif results in defective replication of UV-damaged DNA and hypersensitivity to multiple mutagens.
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