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Reactivity | Human Mouse Rat |
Tested applications | WB IHC IF |
Recommended Dilution | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 |
Calculated MW | 59kDa |
Observed MW | Refer to figures |
Immunogen | Recombinant protein of human RBBP5 |
Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Synonym | RBQ3; SWD1; |
Western blot analysis of extracts of various cell lines, using RBBP5 antibody.
Immunohistochemistry of paraffin-embedded rat Intestine using RBBP5 antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using RBBP5 antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry of paraffin-embedded mouse liver using RBBP5 antibody at dilution of 1:100 (x40 lens).
Immunofluorescence analysis of U20S cell using RBBP5 antibody. Blue: DAPI for nuclear staining.
This gene encodes a ubiquitously expressed nuclear protein which belongs to a highly conserved subfamily of WD-repeat proteins. The encoded protein binds directly to retinoblastoma protein, which regulates cell proliferation. It interacts preferentially with the underphosphorylated retinoblastoma protein via the E1A-binding pocket B. Three alternatively spliced transcript variants that encode different protein isoforms have been described for this gene.
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