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sales@neoscientific.com
+1-888.733.6849
+1-617.299.7367 (Int’l)
+1-888.733.6849
+1-617.299.7367 (Int’l)
| Reactivity | Human Mouse Rat |
| Tested applications | WB IHC IF IP ChIP |
| Recommended Dilution | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:50 - 1:200 ChIP 1:20 - 1:100 |
| Calculated MW | 37kDa |
| Observed MW | Refer to Figures |
| Immunogen | Recombinant protein of human RNF2 |
| Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Concentration | fl |
| Synonym | BAP1; DING; BAP-1; HIPI3; RING2; RING1B; |
Western blot analysis of extracts of various cell lines, using RNF2 antibody.
Immunohistochemistry of paraffin-embedded rat lung using RNF2 antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry of paraffin-embedded human colon using RNF2 antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry of paraffin-embedded human prostate cancer using RNF2 antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry of paraffin-embedded human esophageal cancer using RNF2 antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using RNF2 antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry of paraffin-embedded mouse brain using RNF2 antibody at dilution of 1:100 (x40 lens).
Immunofluorescence analysis of U20S cell using RNF2 antibody. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cell using RNF2 antibody. Green：GFP-RNF168 fusion protein expression for DNA damage marker. Blue: DAPI for nuclear staining.RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.
Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cell using RNF2 antibody. Green：GFP-RNF168 fusion protein expression for DNA damage marker. Blue: DAPI for nuclear staining.RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.
Polycomb group (PcG) of proteins form the multiprotein complexes that are important for the transcription repression of various genes involved in development and cell proliferation. The protein encoded by this gene is one of the PcG proteins. It has been shown to interact with, and suppress the activity of, transcription factor CP2 (TFCP2/CP2). Studies of the mouse counterpart suggested the involvement of this gene in the specification of anterior-posterior axis, as well as in cell proliferation in early development. This protein was also found to interact with huntingtin interacting protein 2 (HIP2), an ubiquitin-conjugating enzyme, and possess ubiquitin ligase activity.
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