ROCK1-Polyclonal Antibodies

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ROCK1

Qty


Total
$300
Catalog #
A0144
Antibody Type
Polyclonal Antibody
Gene ID
6093
Swiss Prot
Q13464
Size
Species
Rabbit
Isotype
IgG
Purity
Affinity purification
Additional Information
ReactivityHuman Mouse Rat
Tested applicationsWB IHC
Recommended DilutionWB 1:500 - 1:2000 IHC 1:50 - 1:200
Calculated MW158kDa
Observed MWRefer to Figures
ImmunogenA synthetic peptide of human ROCK1
Storage BufferStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentrationbdr
SynonymROCK-I; P160ROCK;
Images
  • A0144: image 1

    Western blot analysis of extracts of various cells, using ROCK1 antibody.

Background

ROCK1, also named as p160ROCK and NY-REN-35, belongs to the protein kinase superfamily and AGC Ser/Thr protein kinase family. ROCK1 phosphorylates and activates DAPK3, which then regulates myosin light chain phosphatase through phosphorylation of MYPT1 thereby regulating the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. ROCK1 is required for centromere positioning and centromere-dependent exit from mitosis. It is necessary for apoptotic membrane blebbing. ROCK1 catalyzes the reaction: ATP + a protein = ADP + a phosphoprotein. The antibody is specific to ROCK1.

Protocol

N/A

MSDS
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