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Reactivity | Human Mouse Rat |
Tested applications | WB IHC IF IP |
Recommended Dilution | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 IP 1:20 - 1:50 |
Calculated MW | 160kDa |
Observed MW | Refer to Figures |
Immunogen | Recombinant protein of human ROCK2 |
Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Concentration | dr |
Synonym | ROCK-II; |
Western blot analysis of extracts of various cell lines, using ROCK2 antibody.
Immunohistochemistry of paraffin-embedded Human mammary cancer using ROCK2 antibody at dilution of 1:100 (x400 lens).
Immunohistochemistry of paraffin-embedded Mouse brain using ROCK2 antibody at dilution of 1:100 (x400 lens).
Immunohistochemistry of paraffin-embedded Rat brain using ROCK2 antibody at dilution of 1:100 (x400 lens).
The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho.
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