RPA2-Monoclonal Antibodies

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RPA2

Qty


Total
$220
Catalog #
A0568
Antibody Type
Monoclonal Antibody
Gene ID
6118
Swiss Prot
P15927
Size
Species
Mouse
Isotype
IgG
Purity
Affinity purification
Additional Information
ReactivityHuman
Tested applicationsWB
Recommended DilutionWB 1:500 - 1:1000
Calculated MW29kDa
Observed MWRefer to Figures
ImmunogenA synthetic peptide of human RPA2
Storage BufferStore at 4℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentratione
SynonymREPA2; RPA32
Images
  • A0568: image 1

    Western blot analysis of extracts of HeLa cells tissue, using RPA2 antibody.

Background

RPA70 (HSSB, REPA1, RF-A, RP-A, p70) is a component of a heterotrimeric complex, composed of 70, 32/30 and 14 kDa subunits, collectively known as RPA. RPA is a single stranded DNA binding protein, whose DNA binding activity is believed to reside entirely in the 70 kDa subunit. The complex is required for almost all aspects of cellular DNA metabolism such as DNA replication (1-3), recombination, cell cycle and DNA damage checkpoints, and all major types of DNA repair including nucleotide excision, base excision, mismatch and double-strand break repairs (4-7). In response to genotoxic stress in eukaryotic cells, RPA has been shown to associate with the Rad9/Rad1/Hus1 (9-1-1) checkpoint complex (8). RPA is hyperphosphorylated upon DNA damage or replication stress by checkpoint kinases including ataxia telangiectasia mutated (ATM), ATM and Rad3-related (ATR), and DNA-dependent protein kinase (DNA-PK) (9-11). Hyperphosphorylation may alter RPA-DNA and RPA-protein interactions. In addition to the checkpoint partners, RPA interacts with a wide variety of protein partners, including proteins required for normal replication such as RCF, PCNA and Pol α, and also proteins involved in SV40 replication, such as DNA polymerase I and SV40 large T antigen (10,12).1.Liu, V.F. and Weaver, D.T. (1993) Mol. Cell Biol. 13, 7222-7231.2.Wobbe, C.R. et al. (1987) Proc. Natl. Acad. Sci. USA 84, 1834-1838.3.Fairman, M.P. and Stillman, B. (1988) EMBO J. 7, 1211-1218.4.Wold, M.S. and Kelly, T. (1988) Proc. Natl. Acad. Sci. USA 85, 2523-2527.5.Zhou, B.B. and Elledge, S.J. (2000) Nature 408, 433-439.6.Kastan, M.B. and Bartek, J. (2004) Nature 432, 316-323.7.Sancar, A. et al. (2004) Annu. Rev. Biochem. 73, 39-85.8.Guo, S. et al. (2006) J Biol Chem 281, 21607-16.9.Wu, X. et al. (2005) Oncogene 24, 4728-4735.10.Binz, S.K. et al. DNA Repair (Amst) 3, 1015-1024.11.Nuss, J.E. et al. (2005) Biochemistry 44, 8428-8437.12.Yuzhakov, A. et al. (1999) EMBO J. 18, 6189-6199.

Protocol

N/A

MSDS
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