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Reactivity | Human Mouse Rat |
Tested applications | WB IHC IF |
Recommended Dilution | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
Calculated MW | 29kDa |
Observed MW | Refer to Figures |
Immunogen | Recombinant protein of human RPS3A |
Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Concentration | j |
Synonym | S3A; FTE1; MFTL; |
Western blot analysis of extracts of various cell lines, using RPS3A antibody.
Immunohistochemistry of paraffin-embedded rat brain using RPS3A antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry of paraffin-embedded human liver cancer using RPS3A antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry of paraffin-embedded mouse brain using RPS3A antibody at dilution of 1:100 (x40 lens).
Immunofluorescence analysis of MCF7 cell using RPS3A antibody. Blue: DAPI for nuclear staining.
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S3AE family of ribosomal proteins. It is located in the cytoplasm. Disruption of the gene encoding rat ribosomal protein S3a, also named v-fos transformation effector protein, in v-fos-transformed rat cells results in reversion of the transformed phenotype. This gene is co-transcribed with the U73A and U73B small nucleolar RNA genes, which are located in its fourth and third introns, respectively. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternatively spliced transcript variants have been found for this gene.
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