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Reactivity | Human |
Tested applications | WB IHC IF |
Recommended Dilution | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
Calculated MW | 10kDa |
Observed MW | Refer to Figures |
Immunogen | Recombinant protein of human S100A12 |
Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Synonym | p6; CAGC; CGRP; MRP6; CAAF1; MRP-6; ENRAGE; |
Western blot analysis of extracts of various cell lines, using S100A12 antibody.
Immunohistochemistry of paraffin-embedded human esophageal using S100A12 antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using S100A12 antibody at dilution of 1:100 (x40 lens).
Immunofluorescence analysis of HeLa cell using S100A12 antibody. Blue: DAPI for nuclear staining.
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein is proposed to be involved in specific calcium-dependent signal transduction pathways and its regulatory effect on cytoskeletal components may modulate various neutrophil activities.
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