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Reactivity | Human Mouse Rat |
Tested applications | WB IHC IF |
Recommended Dilution | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
Calculated MW | 100kDa |
Observed MW | Refer to Figures |
Immunogen | Recombinant protein of human SF3B2 |
Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Concentration | j |
Synonym | Cus1; SF3b1; SAP145; SF3B145; SF3b150; |
Western blot analysis of extracts of various cells, using SF3B2 antibody.
Immunohistochemistry of paraffin-embedded human oophoroma using SF3B2 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat liver using SF3B2 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using SF3B2 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using SF3B2 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung cancer using SF3B2 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using SF3B2 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human oophoroma using SF3B2 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human kidney cancer using SF3B2 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using SF3B2 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse Intestine using SF3B2 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using SF3B2 antibody at dilution of 1:100 (40x lens).
Immunofluorescence analysis of MCF7 cell using SF3B2 antibody.
This gene encodes subunit 2 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence-independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. Subunit 2 associates with pre-mRNA upstream of the branch site at the anchoring site. Subunit 2 also interacts directly with subunit 4 of the splicing factor 3b complex. Subunit 2 is a highly hydrophilic protein with a proline-rich N-terminus and a glutamate-rich stretch in the C-terminus.
N/A