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Reactivity | Human Mouse Rat |
Tested applications | WB IHC IF |
Recommended Dilution | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
Calculated MW | 60kDa |
Observed MW | Refer to Figures |
Immunogen | Recombinant protein of human SMAD4 |
Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Concentration | r |
Synonym | JIP; DPC4; MADH4; MYHRS; |
Western blot analysis of extracts of various cell lines, using SMAD4 antibody.
Western blot analysis of extracts from 293 cells and DLD-1 cells using SMAD4 antibody (1:1000).
Immunofluorescence analysis of U20S cell using SMAD4 antibody. Blue: DAPI for nuclear staining.
Immunofluorescence images of SW480 cells showing nuclear and cytoplasmic localization with SMAD4 Antibody 1:200 (top, red).
Immunofluorescence analysis of HeLa cell using SMAD4 antibody. Blue: DAPI for nuclear staining.
This gene encodes a member of the Smad family of signal transduction proteins. Smad proteins are phosphorylated and activated by transmembrane serine-threonine receptor kinases in response to TGF-beta signaling. The product of this gene forms homomeric complexes and heteromeric complexes with other activated Smad proteins, which then accumulate in the nucleus and regulate the transcription of target genes. This protein binds to DNA and recognizes an 8-bp palindromic sequence (GTCTAGAC) called the Smad-binding element (SBE). The Smad proteins are subject to complex regulation by post-translational modifications. Mutations or deletions in this gene have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome.
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