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| Reactivity | Human Mouse Rat |
| Tested applications | WB IHC IF |
| Recommended Dilution | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Calculated MW | 52kDa |
| Observed MW | Refer to figures |
| Immunogen | Recombinant protein of human SMAD9 |
| Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Concentration | r |
| Synonym | PPH2; MADH6; MADH9; SMAD8; SMAD8A; SMAD8B; |
Western blot analysis of extracts of various cell lines, using SMAD9 antibody.
Immunohistochemistry of paraffin-embedded human liver injury using SMAD9 antibody at dilution of 1:100 (x400 lens).
Immunohistochemistry of paraffin-embedded human brain cancer using SMAD9 antibody at dilution of 1:100 (x400 lens).
Immunohistochemistry of paraffin-embedded human kidney using SMAD9 antibody at dilution of 1:100 (x400 lens).
Immunohistochemistry of paraffin-embedded human kidney cancer using SMAD9 antibody at dilution of 1:100 (x400 lens).
Immunohistochemistry of paraffin-embedded mouse brain using SMAD9 antibody at dilution of 1:100 (x400 lens).
Immunohistochemistry of paraffin-embedded mouse kidney using SMAD9 antibody at dilution of 1:100 (x400 lens).
Immunofluorescence analysis of A549 cell using SMAD9 antibody. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of A549 cell using SMAD9 antibody. Blue: DAPI for nuclear staining.
The protein encoded by this gene is a member of the SMAD family, which transduces signals from TGF-beta family members. The encoded protein is activated by bone morphogenetic proteins and interacts with SMAD4. Two transcript variants encoding different isoforms have been found for this gene.
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