|Reactivity||Human Mouse Rat|
|Tested applications||WB IHC ICC IF IP|
|Recommended Dilution||WB 1:500 - 1:2000
IHC 1:50 - 1:200
ICC 1:20 - 1:100
IF 1:20 - 1:100
IP 1:20 - 1:100|
|Observed MW||Refer to Figures|
|Immunogen||Recombinant protein of human SNAP25|
|Storage Buffer||Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.|
Western blot analysis of extracts of various cell lines, using SNAP25 antibody.
Immunofluorescence analysis of HeLa cell using SNAP25 antibody. Blue: DAPI for nuclear staining.
The 25 kDa synaptosome-associated protein (SNAP25) is a target membrane soluble, N-ethylmaleimide-sensitive factor attachment protein receptor (t-SNARE) that is found on neuronal presynaptic membranes. SNAP25 forms a core complex with the SNARE proteins syntaxin and synaptobrevin to mediate synaptic vesicle fusion with the plasma membrane during Ca2+-dependent exocytosis (1). This complex is responsible for exocytosis of the neurotransmitter γ-aminobutyric acid (GABA). Neurotransmitter release is inhibited by proteolysis of SNAP25 by botulinum toxins A and E (2). SNAP25 plays a secondary role as a Q-SNARE involved in endosome fusion; the protein is associated with genetic susceptibility to attention-deficit hyperactivity disorder (ADHD) (3).
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