|Reactivity||Human Mouse Rat|
|Tested applications||WB IHC IF|
|Recommended Dilution||WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200|
|Observed MW||Refer to Figures|
|Immunogen||Recombinant Protein of human SRSF1|
|Storage Buffer||Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.|
|Synonym||ASF; MGC5228; SF2; SF2p33; SRp30a;|
Western blot analysis of extracts of various cells, using SRSF1 antibody.
Immunohistochemistry of paraffin-embedded rat lung using SRSF1 antibody at dilution of 1:100 (x400 lens).
Immunohistochemistry of paraffin-embedded human lung cancer using SRSF1 antibody at dilution of 1:100 (x400 lens).
Immunohistochemistry of paraffin-embedded mouse esophagus using SRSF1 antibody at dilution of 1:100 (x400 lens).
Immunofluorescence analysis of U2OS cell using SFRS1 antibody. Blue: DAPI for nuclear staining.
SF2/ASF is a member of the Ser-Arg-rich (SR) protein family of highly conserved nuclear phosphoproteins involved in pre-mRNA splicing (1). Besides its role in nuclear pre-mRNA splicing, SF2/ASF has been shown to shuttle between the nucleus and cytoplasm, suggesting additional roles in mRNA transport and cytoplasmic events (2). SF2/ASF associates with translating ribosomes and stimulates translation (3). It also activates translation initiation by suppressing the activity of 4E-BP1, which is mediated by SF2/ASF association with mTOR and the phosphatase PP2A (4). More recent studies have demonstrated a role for SF2/ASF in microRNA processing (5).
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