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Reactivity | Human Mouse Rat |
Tested applications | WB IHC IF |
Recommended Dilution | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
Calculated MW | 11kDa |
Observed MW | Refer to Figures |
Immunogen | Recombinant protein of human SUMO2 |
Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Concentration | hop |
Synonym | HSMT3; SMT3B; SUMO3; Smt3A; SMT3H2; |
Western blot analysis of extracts of various cell lines, using SUMO2 antibody.
Immunohistochemistry of paraffin-embedded rat lung using SUMO2 antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using SUMO2 antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry of paraffin-embedded mouse lung using SUMO2 antibody at dilution of 1:100 (x40 lens).
Immunofluorescence analysis of U2OS cell using SUMO2 antibody. Blue: DAPI for nuclear staining.
This gene encodes a protein that is a member of the SUMO (small ubiquitin-like modifier) protein family. It functions in a manner similar to ubiquitin in that it is bound to target proteins as part of a post-translational modification system. However, unlike ubiquitin which targets proteins for degradation, this protein is involved in a variety of cellular processes, such as nuclear transport, transcriptional regulation, apoptosis, and protein stability. It is not active until the last two amino acids of the carboxy-terminus have been cleaved off. Numerous pseudogenes have been reported for this gene. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
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