For quotations, please use our online quotation form, and you may also contact us by
sales@neoscientific.com
+1-888.733.6849
+1-617.299.7367 (Int’l)
+1-888.733.6849
+1-617.299.7367 (Int’l)
Reactivity | Human Mouse Rat |
Tested applications | WB IHC IF |
Recommended Dilution | WB 1:1000 - 1:2000 IHC 1:50 - 1:100 IF 1:100 - 1:300 |
Calculated MW | 12kDa |
Observed MW | Refer to Figures |
Immunogen | A synthetic peptide of human SUMO3 |
Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Concentration | hop |
Synonym | SMT3A; Smt3B;SMT3H1;SUMO-3; |
Western blot analysis of extracts of MCF7 cell line, using SUMO3 antibody.
Immunohistochemistry of paraffin-embedded mouse brain using SUMO3 antibody.
Immunohistochemistry of paraffin-embedded rat brain using SUMO3 antibody.
Immunohistochemistry of paraffin-embedded human prostate using SUMO3 antibody.
Immunofluorescence analysis of A549 cell using SUMO3 antibody. Blue: DAPI for nuclear staining.
Small ubiquitin-related modifier 1, 2 and 3 (SUMO-1, -2 and -3) are members of the ubiquitin-like protein family (1). The covalent attachment of the SUMO-1, -2 or -3 (SUMOylation) to target proteins is analogous to ubiquitination. This post-translational modification is a reversible, multi-step process that is initiated by cleaving a precursor protein to a mature protein. Mature SUMO-1, -2 or -3 is then linked to the activating enzyme E1, conjugated to E2 and in conjunction with E3, SUMO-1, -2 or -3 is ligated to the target protein (2). Ubiquitin and the individual SUMO family members are all targeted to different proteins with diverse biological functions. Ubiquitin predominantly regulates degradation of its target (1). In contrast, SUMO-1 is conjugated to RanGAP, PML, p53 and IκB-α to regulate nuclear trafficking, formation of subnuclear structures, regulation of transcriptional activity and protein stability (3-7). SUMO-2/-3 forms poly-(SUMO) chains, is conjugated to topoisomerase II and APP, regulates chromosomal segregation and cellular responses to environmental stress, and plays a role in the progression of Alzheimer disease (8-11).
N/A