TRIM21-Monoclonal Antibodies

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TRIM21

Qty


Total
$220
Catalog #
A1957
Antibody Type
Polyclonal Antibody
Gene ID
6737
Swiss Prot
P19474
Size
Species
Rabbit
Isotype
IgG
Purity
Affinity purification
Additional Information
ReactivityHuman Mouse
Tested applicationsWB IHC IF
Recommended DilutionWB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:10 - 1:100
Calculated MW54kDa
Observed MWRefer to Figures
ImmunogenRecombinant protein of human TRIM21
Storage BufferStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentrationl
SynonymRNF81; RO52; SSA; SSA1;
Images
  • A1957: image 1

    Western blot analysis of extracts of various cell lines, using TRIM21 antibody.

  • A1957: image 2

    Immunohistochemistry of paraffin-embedded mouse heart using TRIM21 antibody at dilution of 1:100 (x40 lens).

  • A1957: image 3

    Immunofluorescence analysis of U2OS cell using TRIM21 antibody. Blue: DAPI for nuclear staining.

Background

TRIM21 has multiple N-terminal zinc finger motifs(has E3 ligase activity), a central leucine zipper, and a potential N-glycosylation site. TRIM3 is a E3 ubiquitin-protein ligase. It can form a ubiquitin ligase complex with E2 UBE2D2, which function not only for the uniquitination of USP4 and IKBKB but also for its self-ubiquitination. It has a role in the regulation of the cell cycle progression and could enhance the decapping activity of DCP2. It can exist in all mammalian cells as a ribonucleoprotein particle , which composed of a single polypeptide and 1 of 4 small RNA molecules.

Protocol

N/A

MSDS
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