|Reactivity||Human Mouse Rat|
|Tested applications||WB IHC IF|
|Recommended Dilution||WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:10 - 1:100|
|Observed MW||Refer to Figures|
|Immunogen||Recombinant protein of human TUBA4A|
|Storage Buffer||Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.|
|Synonym||TUBA4A ; Tubulin alpha 4A chain; tubulin; alpha 4a; TUBA1; Tubulin alpha 1 chain; FLJ30169; α-Tubulin;|
Western blot analysis of extracts of various cell lines, using TUBA4A antibody.
Immunofluorescence analysis of U2OS cell using TUBA4A antibody. Blue: DAPI for nuclear staining.
Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin.
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