UBB-Polyclonal Antibodies

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UBB

Qty


Total
$380
Catalog #
A0162
Antibody Type
Polyclonal Antibody
Gene ID
7314
Swiss Prot
P0CG47
Size
Species
Rabbit
Isotype
IgG
Purity
Affinity purification
Additional Information
ReactivityHuman Mouse Rat
Tested applicationsWB IHC ICC FC
Recommended DilutionWB 1:1000 - 1:2000 IHC 1:100 - 1:200 ICC 1:100 - 1:200 FC 1:50 - 1:200
Calculated MW8kDa
Observed MWRefer to Figures
ImmunogenA synthetic peptide of human UBB
Storage BufferStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentrationbi
SynonymUBB;FLJ25987;MGC8385
Images
  • A0162: image 1

    Western blot analysis of extracts of various cells, using UBB antibody.

Background

Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thiolester complex with the ubiquitin-activating enzyme (UBE1 or E1). The activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2, and then from E2 to ubiquitin ligase E3 for final delivery to the ε-amino group of the target protein lysine residue (1-3). Combinatorial interactions of different E2 and E3 proteins result in substrate specificity (4). UBE1 has two isofoms: UBE1a is a nuclear protein of 117 kDa while UBE1b is a nuclear and cytoplasmic protein of 110 kDa (5).

Protocol

N/A

MSDS
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