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Reactivity | Human Mouse Rat |
Tested applications | WB IHC ICC FC |
Recommended Dilution | WB 1:1000 - 1:2000 IHC 1:100 - 1:200 ICC 1:100 - 1:200 FC 1:50 - 1:200 |
Calculated MW | 8kDa |
Observed MW | Refer to Figures |
Immunogen | A synthetic peptide of human UBB |
Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Concentration | bi |
Synonym | UBB;FLJ25987;MGC8385 |
Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thiolester complex with the ubiquitin-activating enzyme (UBE1 or E1). The activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2, and then from E2 to ubiquitin ligase E3 for final delivery to the ε-amino group of the target protein lysine residue (1-3). Combinatorial interactions of different E2 and E3 proteins result in substrate specificity (4). UBE1 has two isofoms: UBE1a is a nuclear protein of 117 kDa while UBE1b is a nuclear and cytoplasmic protein of 110 kDa (5).
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