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sales@neoscientific.com
+1-888.733.6849
+1-617.299.7367 (Int’l)
+1-888.733.6849
+1-617.299.7367 (Int’l)
| Reactivity | Human Mouse Rat |
| Tested applications | WB IHC |
| Recommended Dilution | WB 1:500 - 1:2000 IHC 1:50 - 1:200 |
| Calculated MW | 17kDa |
| Observed MW | Refer to Figures |
| Immunogen | Recombinant protein of human UBE2B |
| Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Synonym | HR6B; UBC2; HHR6B; RAD6B; E2-17kDa; |
Western blot analysis of extracts of various cell lines, using UBE2B antibody.
Immunohistochemistry of paraffin-embedded rat lung using UBE2B antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry of paraffin-embedded human liver cancer using UBE2B antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry of paraffin-embedded human esophageal cancer using UBE2B antibody at dilution of 1:100 (x40 lens).
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair. Its protein sequence is 100% identical to the mouse, rat, and rabbit homologs, which indicates that this enzyme is highly conserved in eukaryotic evolution.
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