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sales@neoscientific.com
+1-888.733.6849
+1-617.299.7367 (Int’l)
+1-888.733.6849
+1-617.299.7367 (Int’l)
| Reactivity | Human Mouse Rat |
| Tested applications | WB IHC |
| Recommended Dilution | WB 1:1000 - 1:2000 IHC 1:50 - 1:100 |
| Calculated MW | 18kDa |
| Observed MW | Refer to Figures |
| Immunogen | Recombinant protein of human UBE2L3 |
| Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Concentration | fijl |
| Synonym | E2-F1; L-UBC; UBCH7; UbcM4; |
Western blot analysis of extracts of various cell lines, using UBE2L3 antibody.
Immunohistochemistry of paraffin-embedded rat heart using UBE2L3 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using UBE2L3 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse brain using UBE2L3 antibody at dilution of 1:100 (40x lens).
The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s) and ubiquitin-protein ligases (E3s). This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is demonstrated to participate in the ubiquitination of p53, c-Fos, and the NF-kB precursor p105 in vitro. Several alternatively spliced transcript variants have been found for this gene.
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