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sales@neoscientific.com
+1-888.733.6849
+1-617.299.7367 (Int’l)
+1-888.733.6849
+1-617.299.7367 (Int’l)
| Reactivity | Human Mouse Rat |
| Tested applications | WB IHC IF |
| Recommended Dilution | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Calculated MW | 123kDa |
| Observed MW | Refer to Figures |
| Immunogen | A synthetic peptide of human UPF1 |
| Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Concentration | bj |
| Synonym | FLJ43809; FLJ46894; HUPF1; KIAA0221; NORF1; RENT1; pNORF1; |
Western blot analysis of extracts of various cell lines, using UPF1 antibody.
Immunohistochemistry of paraffin-embedded human liver cancer using UPF1 antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry of paraffin-embedded mouse brain using UPF1 antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry of paraffin-embedded mouse heart using UPF1 antibody at dilution of 1:100 (x40 lens).
Immunofluorescence analysis of HeLa cell using UPF1 antibody. Blue: DAPI for nuclear staining.
Upf1 was identified as an active component in nonsense-mediated decay (NMD), an mRNA surveillance mechanism in eukaryotic cells that degrades mRNAs containing premature termination codons (1). Upf1 was found to be an ATP-dependent RNA helicase in the cytoplasm (2) and was later shown to be a component of cytoplasmic P-bodies (3). Upf1 phosphorylation mediates the repression of translation that accompanies NMD, allowing mRNA accessibility to the NMD machinery (4). Two other active components of NMD, Upf2 and Upf3, were also identified and described as having perinuclear and nucleocytoplasmic localization, respectively (5).
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