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Reactivity | Human Mouse Rat |
Tested applications | WB IHC IF |
Recommended Dilution | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:10 - 1:100 |
Calculated MW | 64kDa |
Observed MW | Refer to Figures |
Immunogen | Recombinant protein of human cdc45 |
Storage Buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Synonym | CDC45L; CDC45L2; PORC-PI-1; |
Western blot analysis of extracts of various cells, using cdc45 antibody.
Immunohistochemistry of paraffin-embedded mouse stomach using CDC45 antibody at dilution of 1:100 (x40 lens).
Immunohistochemistry of paraffin-embedded human stomach using CDC45 antibody at dilution of 1:100 (x40 lens).
Immunofluorescence analysis of HeLa cell using CDC45 antibody. Blue: DAPI for nuclear staining.
The protein encoded by this gene was identified by its strong similarity with Saccharomyces cerevisiae Cdc45, an essential protein required to the initiation of DNA replication. Cdc45 is a member of the highly conserved multiprotein complex including Cdc6/Cdc18, the minichromosome maintenance proteins (MCMs) and DNA polymerase, which is important for early steps of DNA replication in eukaryotes. This protein has been shown to interact with MCM7 and DNA polymerase alpha. Studies of the similar gene in Xenopus suggested that this protein play a pivotal role in the loading of DNA polymerase alpha onto chromatin. Multiple alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2010]
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