ARFIP2 Human Recombinant produced in E. coli is a single polypeptide chain containing 364 amino acids (1-341) and having a molecular mass of 40.2 kDa.ARFIP2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Arfaptin 2 (ARFIP2) is a Rac1 binding protein essential for Rac-mediated actin polymerization and the succeeding formation of membrane ruffles and lamellipodia. ARFIP2 is a putative target protein of ADP-ribosylation factor. ARFIP2 is also involved in membrane ruffling. ARFIP2 expression is increased at sites of neurodegeneration. In addition, ARFIP2 interacts with the ADP ribosylation factor ARF6, a GTPase which associates with the plasma membrane and intracellular endosome vesicles, in a GTP dependent mode. Furthermore, Arfaptin 2 controls the aggregation of mutant Huntingtin protein by weakening proteasome function.
ADP-ribosylation factor interacting protein 2, partner of RAC1 (arfaptin 2), Partner of RAC1, Protein POR1, arfaptin-2, POR1.
Sterile Filtered colorless solution.
The ARFIP2 solution (0.25mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 1mM DTT and 40% glycerol.
Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.