Adenosine 5" Triphosphate Sulfurylase Yeast Recombinant produced in E.Coli is a non-glycosylated, polypeptide chain containg 511 amino acids and having a Mw of 57.7 kDa. Adenosine 5" Triphosphate Sulfurylase Yeast Recombinant catalyzes the activation of sulfate by transferring sulfate to the adenine monophosphate moiety of ATP to form adenosine 5
ATP sulphurylase synthesizes adenosine 5’-sul-phatophosphate from ATP and inorganic SO42-. This is the first reaction of a two step sequence in the formation of “active sulphate”, adenosine 3’-phosphate5’-sulphatophosphate, which is a sulphate donor for a wide variety of compounds and is also involved in the reduction of sulphate.
Sterile Filtered White lyophilized (freeze-dried) powder.
The ATP sulphurylase protein was lyophilized after dialysis against lyophilized from 10mM NaP buffer, 100mM NaCl, 10mM Lactose, 1% PEG pH 7.5 and 0.75mM DTT.
Spin vial before opening. Reconstitute ATP sulphurylase with 5mM NaP pH-7.5 & 0.75mM DTT at a concentration ranging from 0.1mg – 1mg per ml. Can be diluted further into other aqueous buffers. pH range between 7.0 – 8.5 is best.
Lyophilized Sulfate adenylate transferase althoµgh stable at 4°C for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution ATPS should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.