b NGF Human-Beta-Nerve Growth Factor

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b NGF Human

Qty


Total
$39
Catalog #
CYPS-586
Size
Description
Nerve Growth Factor-beta Human Recombinant produced in E.Coli is a non-covalently disulfide-linked homodimer, non-glycosylated, polypeptide chain containing 2 identical 121 amino acids with a molecular weight of two 13.6 kDa polypeptide monomers.The NGF-b is purified by proprietary chromatographic techniques.
Additional Information
IntroductionNGF-beta has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene's expression is associated with allergic rhinitis.
SynonymsBeta Polypeptide, NGF, NGFB, HSAN5, Beta-NGF, MGC161426, MGC161428.
SourceEscherichia Coli.
Physical AppearanceSterile Filtered White lyophilized (freeze-dried) powder.
FormulationThe beta-NGF protein was lyophilized from a 0.2
SolubilityIt is recommended to reconstitute the lyophilized NGF-b in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
StabilityLyophilized Beta-NGF althoµgh stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution NGF-Beta should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Amino Acid SequenceMSSSHPIFHRG
EFSVCDSVSV
WVGDKTTATD
IKGKEVMVLG
EVNINNSVFK
QYFFETKCRD
PNPVDSGCRG
IDSKHWNSYC
TTTHTFVKAL
TMDGKQAAWR
FIRIDTACVC
VLSRKAVRRA.
PurityGreater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Biological ActivityThe ED50, calculated by its ability to stimulate chick E9 DRG neurite outgrowth was found to be < 1.0ng/ml, corresponding to a specific activity of > 1,000,000units/mg.
UsageNeoScientific's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drµgs, agricultural or pesticidal products, food additives or household chemicals.
Background

N/A

Protocol

N/A

MSDS
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